Description :
- Portrayal of ideal cooling rates and ideal conventions for both porcine semen (slow freezing) and oocytes (slow freezing and vitrification)
- Decide the impact of cryopreservation strategies on individual sperm cells motility boundaries (CASA)
- Utilization of a clever technique to tweak ice arrangement and recrystallization utilizing a mix of Ice Restricting Proteins and cooling rates to further develop endurance and improvement of frozen-defrosted semen and oocytes
- Apply cryomicroscopy and microscopy methods to picture controlled cooling, ice arrangement and freezing and survey cryo-harm during freezing-defrosting cycles
Location :
- Centre for Genetic Resources, Wageningen University and Research (WUR), the Netherlands
Main Supervisor :
- Dr. Julie Lamy
- E-mail : Julie.lamy@wur.nl
Participating researchers :
- Dr. Henri Woelders (WUR)
- Dr. Louisa Zak (Topigs Norsvin)
Results :
- Improvement of cryopreservation strategies for porcine semen
- Unraveling of cryo-injury instruments during freezing of porcine semen and oocytes
- Normalized technique for the cryopreservation of juvenile and mature porcine oocytes
Planned secondments: (Host, Supervisor, timing, length, and purpose) :
- Topigs Norsvin, Louisa Zak, M16, 3 M, Porcine semen collection and processing
- LUH, Birgit Glasmacher, M26, 3 M, Applied cryomicroscopy techniques to visualize cryodamage in pig oocytes and embryos
- SCBI, Pierre Comizzoli, M32, 2 M, Learning advanced methods for long term preservation and genetic resource banking
Apply :
- You Can apply by Send your CV, motivation letter and names and contact details for referees by e-mail to the Main Supervisor.Julie.lamy@wur.nl
