Description :
- Create and test a tool to enable a multiphoton cryo-microscope to observe phase shifts in animal germplasm under directional freezing.
- The outcome of cryopreservation varies greatly when ice crystals grow out of control during spontaneous freezing.
- To provide optimal temperature control and suitability for multiphoton microscopy, the design, manufacture, and validation of a directional freezing cryostage will be part of this process.
- LLS Rowiak will actively assist in conducting the system’s validation.
- Application of reproductive cells and the appropriate fluorescent dyes (such as Hoechst33342, CellLight Plasma Membrane-CFP) will be used to monitor the integrity of the cell’s internal structure and any membrane alterations that occur during cryopreservation.
- Create sophisticated picture processing and analysis software.
- Images taken using a multiphoton cryo-microscope will be utilized to develop software for image processing and analysis. This particular objective will be conducted in cooperation with NURE.
- Investigate samples with multiphoton cryo-microscope during freezing and thawing process including image analysis. The effect of freezing parameters (temperature gradient, CPA/VA type, cooling rate) on the integrity of reproductive cells will be analyzed.
Location :
- Institute for Multiphase Processes, Leibniz University Hannover, Germany
Main Supervisor :
- Prof. Birgit Glasmacher
- E-mail : glasmacher@imp.uni-hannover.de
Participating researchers :
- Prof. Oleg Avrunin (NURE)
- Dr. Birgitta Stolze (LLS Rowiak)
- Dr. Tim Rittinghaus (LUH)
Results :
- Better understanding of the effects of chilling and warming on ice formation, devitrification, and ice recrystallization in sperm and oocytes
- With a directed freezing cryostage, a working multiphoton-cryo-microscope has been validated.
- Software for image processing that characterizes cooling and warming samples
Planned secondments: (Host, Supervisor, timing, length, and purpose)
- LLS ROWIAK, Stolze, M12, 2 M, Training in multiphoton microscopy and recommendations for a cryostage
- NURE, Avrunin, M24, 2 M, Training in image analysis techniques
How to Apply :
- You Can apply by Send your CV, motivation letter and names and contact details for referees by e-mail to the Main Supervisor.
